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Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution. Notice that the side chain has three methylene groups, in order that though the terminal amino group might be charged underneath physiological conditions, the aspect chain does have important hydrophobic character. Incubate the membrane for 20 min after which wash with DMF to take away uncoupled amino acids.